浊度、pH值及温度对光谱法检测海水中藻红蛋白含量的影响

Effects of turbidity,pH and temperature on the spectrosc opy method in the detection of phycoerythrin contents in seawater

摘要:

为验证水的浊度、pH值 及温度等因素对藻红蛋白(PE)含量检测的影响,以浓度范围5~180mg /L PE配置 的标准液为研究对象,采集不同浓度标准液的可见光吸收光谱,运用偏最小二乘法(PLS) 建立不同谱区的PE定量分析模型。实验结果表明,480~570nm谱区所 建模型相关性最高,相关系 数R²可达0.993,预测结果误差最小 ；同时,对0~90NTU不同浊度的PE标准液进行浊度补偿分析,建 立了不同分析模型下的浊度补偿函数； 分析了温度9.7~52.8 ℃, pH在4.2范围 内PE的光谱特 性,当温度在40℃以下时PE的光谱特性基本不发生变化,而pH 在6.0~9.0变化时影响可以忽略。本文研究可为海水中PE含量的在线 检测参考。

Abstract:

Phycoerythrin (PE) is an important indicator which measures the algae e utrophication in water.In order to verify the influence of turbidity,pH and temperature on determination of PE,the visible absorption spec tra of with concentration the standard solutions of PE in the range of 5~180mg/ L are collected.The partial least squares (PLS) algorithm is used to establish t he quantitative analysis models for different spectral regions.The experimental results show that the model in the spectral region of 480~570nm ha ve the highest correlation and the minimum error.By analyzing the phycoerythrin solutions different turbidi ty of 0~90NTU,the turbidity compensation function for the different spectral region is established by analys is model.By analyzing the PE spectral characteristics in the temperature range o f 9.7~52.8℃,pH of 4.2~10.5,the PE spectral characteris tics almost do not change when the temperature is below 40℃ and the pH is in the range 6.0~9.0.It can lay the fou ndation for on-line detection of PE content in seawater.