基于光热效应与表面等离子体共振的生物分子检测技术

Detection technology of biomolecular based on photothermal effect and surface plasmon resonance

摘要:

针对生物标志物的高灵敏度检测，提出了一种基于光热效应和表面等离子体共振（Surface plasmon resonance，SPR）的生物分子检测技术。该技术在SPR光强模式下，通过泵浦光激发显色反应溶液的光热效应，使得溶液折射率发生变化，从而引起探测光光强发生变化，其变化值与溶液浓度正相关。文中以乳酸脱氢酶（lactate dehydrogenase，LDH）为例，利用显色反应使得LDH显色，结合光热效应和SPR的光强模式实现了LDH的定量检测，检测限为 0.298 mU/mL。

Abstract:

A biomolecule detection technology based on photothermal effect and surface plasmon resonance (SPR) is proposed for high-sensitivity detection of biomarkers. In SPR intensity mode, photothermal effect of the color reaction solution is induced by pump light, causing a change in the refractive index of the solution and a change of the probe light intensity, which is positively correlated with the solution concentration. Taking lactate dehydrogenase (LDH) as an example in this work, a colorimetric reaction was used to color LDH, and the quantitative detection of LDH was achieved by combining photothermal effect and SPR technology with light intensity mode, with a detection limit of 0.298 mU/mL.